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Anti-neurexin-3α antibody-associated encephalitis is rare in clinical practice. It often has a history of pre-infection. It is characterized by abnormal mental behavior, seizures, decreased consciousness, cognitive and sleep disorders, movement disorder, central hypoventilation and autonomic nervous dysfunction. Among them, dyskinesias are mainly involuntary movements of the mouth, face and limbs, dystonia, myoclonic seizures and other manifestations of increased movement. Parkinson′s symptoms manifested as decreased movement are rarely reported. A encephalitis patient with positive anti-neurexin-3α antibody is reported, who is a young female, mainly with parkinsonism such as slow movement, unsteady walking, difficulty in starting and turning around, and inability to hold things in both upper limbs, accompanied by abnormal mental behavior and cognitive dysfunction. After treatment with methylprednisolone and intravenous immunoglobulin, the prognosis is good.
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Objective To investigate diagnosis and treatment of venous cerebral infarction.Methods The reasons, clinical findings, imaging characters, treatment methods and prognosis of 19 patients diagnosed venous cerebral infarction were analyzed.Results The most frequent symptoms were headache, focal deficits, epilepsy, increased CSF pressure.Among 19 cases, multiple cerebral lesions were found in 12 cases, lobes lesions in18 cases, cerebral infarction with hemorrhage in 3 cases. After various treatments, 16 patients were discharged and one patient died and 2 patients gave up treatment. Conclusions Venous cerebral infarction has complicated causes, non-specific chinical manifestations and characteristic MR images. Anticoagulation and thrombolysis treatment in early stage may have good therapeutic effect.
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Objective To identify the property and implications of dendritic cells of peripheral blood monocytes (PBMC) in patients with persistent HBV infection. Methods The PBMC from 18 patients with persistent HBV infection and 10 healthy subjects were incubated and induced into mature dendritic cells (DC) in the completed medium containing GM CSF、IL 4、FLt3 L and TNF ? cytokines. The flow cytometric analysis was employed to detect the expression of surface markers on DC. ELISA test was used to determine the level of interleukin 12 (IL 12) and IL 10 cytokines produced by DCs. The stimulatory capacity of DC were also determined in allogenic mixed leukocyte reaction (MLR). Results A typical morphology of DCs was observed when the PBMCs from healthy subjects and HBV infected patients were induced for 6 7 day in vitro incubation, but there were some significant differences of DCs as follows: (1) the proliferation ability and number of DCs significantly decreased, in particular, the expression levels of HLA DR,CD80(B7 1),CD86(B7 2) and CD1? on DC surface were simultaneously much lower in patients compared to healthy subjects ( P
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Purpose:To evaluate the antitumor effects of human cytokine-induced killer (CIK) cells from healthy donors and patients with primary hepatocellular carcinoma (HCC) in vitro and in vivo .Methods:Peripheral blood mononuclear cells (PBMC) from healthy donors and patients were induced to become CIK cells by in vitro incubation. The CIK cells were identified at different intervals by flow cytometry analysis. The cytotoxicity of CIK cells against autologous primary HCC was determined by 51Cr Release Assays. The antitumor activity of the CIK, LAK and PBMC cells were evaluated in Balb/c nude mice bearing BEL-7402liver cancer.Results:The flow cytometry analysis showed that CIK cells from patients with HCC proliferated and expanded more than 300-fold in vitro incubation by day 28;the percentage of CD3+CD56+ cells increased from 0.23% on day 0 to 17.8% on day 21. CIK cells generated from patients with HCC patients possessed a higher antitumor cytotoxic activity on autologous HCC cells in vitro than autologous PBMC. In addition, CIK cells had a stronger suppressive effect on the tumor growth in Balb/c nude mice bearing BEL-7402 tumor than LAK cells (median inhibitory rates 84.7% vs 52.8%, P
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Whole blood samples were collected from 316 Uigur subjects inhabiting Kashi City, Sinkiang Uigur Autonomous Region, and their genomic DNA was extracted using QIAgen Blood Kit. DNA fragment spanning CCR5?32 and CCR5m303 mutation points were amplified by PCR and genotyped by directed electrophoresis and RFLP with HinCII respectively. In all 316 samples only 22 heterozygotes of CCR5?32 were found, in accordance with Hardy Weinberg equilirium. No CCR5m303 mutation was found. No difference existed between male and female individuals. The frequency of CCR5?32 in Uigur population is 3.48%, similar to Medi Asia ethnic groups (higher than that of Chinese Han people and lower than that of Caucasian people), i.e, in continuity regarding district distribution. Due to its slow down effect on clinical course of AIDS , high frequency of CCR5?32 is highly significant in prevention and treatment of AIDS in Uigur population.
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It was reported that 249I 280M haplotype of HIV coreceptor CX 3 CR1 was associated with accelerated AIDS progression. In this study, genomic DNA of 119 Uigur subjects (98 normal, 21 HIV 1 infected) was purified from PBMC, and 249I and 280M allelic frequencies were identified by PCR RFLP. All data were tested by ? 2 analysis.It was found that allelic frequency of 249I and 280M was 15 8% and 13 8%, respectively, and there was no difference between normal and HIV infected groups. Both population and sex distribution were in accordance with Hardy Weinberg equilibrium. Frequency of 249I280M haplotype was 13 8%, close to that of Caucasian people. No linkage was found between CX 3 CR1 and CCR5?32, but strong linkage existed between 249I and 280M ( P
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Forty five Han individuals were enrolled for the investigation of the polymorphism property of HIV 1 associated SDF 1? in Chinese Han nationality. Four exons of SDF 1? were cloned by PCR amplification using 4 pairs of primers, then sequenced respectively. DNAstar software was employed to analyze the sequencing results. One SNP and one thymidine deletion were identified in the coding region of SDF1 ?. The SNP was G→T at 192 with a frequency of 8.9%, resulting in replacing lysine to asparagine. The thymidine deletion was at 100 (100?T), which caused frame shift from the 34th amino acid and ending at the 59th amino acid. Both of them were discovered for the first time, and their influences on HIV 1 infection and AIDS progression remain to be explored.